nco i hf (New England Biolabs)
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New England Biolabs
nco i hf
Nco I Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 3184 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nco+i+hf/NcoI/pmc12813566-71-20-22
Average 98 stars, based on 3184 article reviews
Nco I Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 3184 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nco+i+hf/NcoI/pmc12813566-71-20-22
Average 98 stars, based on 3184 article reviews
nco i hf - by Bioz Stars,
2026-10
98/100 stars
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Plasmid Preparation:Article Title: A growth-coupling strategy for improving the stability of terpenoid bioproduction in Escherichia coli Article Snippet: Whole plasmid sequencing was performed by Plasmidsaurus using Oxford Nanopore Technology with custom analysis and annotation. .. For visualisation of plasmid on an agarose gel, plasmids were first digested with Article Title: An RNA-based system to study hepatitis B virus replication and evaluate antivirals. Article Snippet: Yingpu Yu†, William M. Schneider†*, Maximilian A. Kass, Eleftherios Michailidis‡, Ashley Acevedo, Ana L. Pamplona Mosimann§, Juliano Bordignon§, Alexander Koenig, Christine M. Livingston, Hardeep van Gijzel, Yi Ni, Pradeep M. Ambrose, Catherine A. Freije, Mengyin Zhang, Chenhui Zou, Mohammad Kabbani, Corrine Quirk, Cyprien Jahan||, Xianfang Wu¶, Stephan Urban, Shihyun You, Amir Shlomai#**, Ype P. de Jong**, Charles M. Rice* Article Title: Cis -regulatory interfaces reveal the molecular mechanisms underlying the notochord gene regulatory network of Ciona Article Snippet: .. The Foxa.a::GFP fragment was then excised using Xho I and Agarose Gel Electrophoresis:Article Title: A growth-coupling strategy for improving the stability of terpenoid bioproduction in Escherichia coli Article Snippet: Whole plasmid sequencing was performed by Plasmidsaurus using Oxford Nanopore Technology with custom analysis and annotation. .. For visualisation of plasmid on an agarose gel, plasmids were first digested with Article Title: Methods for producing polypeptides in protease-deficient mutants of Article Snippet: .. Genomic DNA from each of the 15 transformants was extracted as described in Example 11 and 2 μg of each were digested with 20 units of Polymerase Chain Reaction:Article Title: Characterization of RNA Helicase Genes in Ustilago maydis Reveals Links to Stress Response and Teliospore Dormancy Article Snippet: The uded1 expression construct was created using the modified p123 vector (p123 + crg1). uded1 was PCR amplified with primers UMAG_04080_NcoI-F and UMAG_04080_NotI_R from U. maydis gDNA using Phusion High Fidelity DNA Polymerase. .. Both p123 + crg1 and PCR products were digested with Article Title: An RNA-based system to study hepatitis B virus replication and evaluate antivirals. Article Snippet: Yingpu Yu†, William M. Schneider†*, Maximilian A. Kass, Eleftherios Michailidis‡, Ashley Acevedo, Ana L. Pamplona Mosimann§, Juliano Bordignon§, Alexander Koenig, Christine M. Livingston, Hardeep van Gijzel, Yi Ni, Pradeep M. Ambrose, Catherine A. Freije, Mengyin Zhang, Chenhui Zou, Mohammad Kabbani, Corrine Quirk, Cyprien Jahan||, Xianfang Wu¶, Stephan Urban, Shihyun You, Amir Shlomai#**, Ype P. de Jong**, Charles M. Rice* Article Title: Large-scale experimental assessment of variant effects on the structure and function of the citrate transporter SLC13A5 Article Snippet: The attB_Strp_HA_Ct-SLC13A5 plasmid was constructed with the attB backbone vector and the SLC13A5 with C-terminal STREP II and HA tags as insert using the GenBuilder Cloning Kit (GenScript, L00701 ) according to the manufacturer’s protocol. .. The attB backbone was made by the removal of enhanced green fluorescent protein (EGFP) from attB_EGFP with Transformation Assay:Article Title: An RNA-based system to study hepatitis B virus replication and evaluate antivirals. Article Snippet: Yingpu Yu†, William M. Schneider†*, Maximilian A. Kass, Eleftherios Michailidis‡, Ashley Acevedo, Ana L. Pamplona Mosimann§, Juliano Bordignon§, Alexander Koenig, Christine M. Livingston, Hardeep van Gijzel, Yi Ni, Pradeep M. Ambrose, Catherine A. Freije, Mengyin Zhang, Chenhui Zou, Mohammad Kabbani, Corrine Quirk, Cyprien Jahan||, Xianfang Wu¶, Stephan Urban, Shihyun You, Amir Shlomai#**, Ype P. de Jong**, Charles M. Rice* Bacteria:Article Title: An RNA-based system to study hepatitis B virus replication and evaluate antivirals. Article Snippet: Yingpu Yu†, William M. Schneider†*, Maximilian A. Kass, Eleftherios Michailidis‡, Ashley Acevedo, Ana L. Pamplona Mosimann§, Juliano Bordignon§, Alexander Koenig, Christine M. Livingston, Hardeep van Gijzel, Yi Ni, Pradeep M. Ambrose, Catherine A. Freije, Mengyin Zhang, Chenhui Zou, Mohammad Kabbani, Corrine Quirk, Cyprien Jahan||, Xianfang Wu¶, Stephan Urban, Shihyun You, Amir Shlomai#**, Ype P. de Jong**, Charles M. Rice* Over Expression:Article Title: The EamA metabolite transporter does not affect antileishmanial drug susceptibility Article Snippet: .. LINF_020008400 overexpression in L. infantum was accomplished using the pLEXSY system. pLEXSY hyg2.0 was digested using Not I-HF® (NEB) and Purification:Article Title: The EamA metabolite transporter does not affect antileishmanial drug susceptibility Article Snippet: .. LINF_020008400 overexpression in L. infantum was accomplished using the pLEXSY system. pLEXSY hyg2.0 was digested using Not I-HF® (NEB) and Gel Extraction:Article Title: The EamA metabolite transporter does not affect antileishmanial drug susceptibility Article Snippet: .. LINF_020008400 overexpression in L. infantum was accomplished using the pLEXSY system. pLEXSY hyg2.0 was digested using Not I-HF® (NEB) and Amplification:Article Title: Large-scale experimental assessment of variant effects on the structure and function of the citrate transporter SLC13A5 Article Snippet: The attB_Strp_HA_Ct-SLC13A5 plasmid was constructed with the attB backbone vector and the SLC13A5 with C-terminal STREP II and HA tags as insert using the GenBuilder Cloning Kit (GenScript, L00701 ) according to the manufacturer’s protocol. .. The attB backbone was made by the removal of enhanced green fluorescent protein (EGFP) from attB_EGFP with Membrane:Article Title: Methods for producing polypeptides in protease-deficient mutants of Article Snippet: .. Genomic DNA from each of the 15 transformants was extracted as described in Example 11 and 2 μg of each were digested with 20 units of Derivative Assay:Article Title: Cis -regulatory interfaces reveal the molecular mechanisms underlying the notochord gene regulatory network of Ciona Article Snippet: .. The Foxa.a::GFP fragment was then excised using Xho I and Construct:Article Title: Cis -regulatory interfaces reveal the molecular mechanisms underlying the notochord gene regulatory network of Ciona Article Snippet: .. The Foxa.a::GFP fragment was then excised using Xho I and |